Engineer EVs to deliver cargo to cells expressing PD-1 on their surface using the streamlined XStamp Pro PD-1 workflow—works with already-purified EVs

Speed production of target-specific EVs with the next generation of XStamp technology
Streamline engineering by working directly with purified EVs—no expression vector or transfection required
Save hands-on time with a simple workflow—just incubate EVs with the XStamp ScFv for one hour and isolate target-specific EVs
Easily generate multiple targeting variants for quick screening studies
Compatible with any EV loading protocol

purified EVs XStamp Pro 

The XStamp™ Pro anti-PD-1 EV Targeting Kit enables streamlined generation of extracellular vesicles (EVs) specific for cells that express PD-1 on their surface. Just decorate the outside of already isolated EVs with the XStamp Pro anti-PD-1 ScFv and then isolated your targeting EVs (see all of the targeting options in table Related Products below). Save time and simplify your EV engineering workflow with XStamp Pro.

While conventional methods for creating target-specific EVs are highly robust (see XStamp Cloning and Expression Lentivector), they involve cloning and manipulation of the producer cells which can be challenging, especially if the cells are hard to transfect. To streamline this process and help researchers more quickly and easily create EVs that preferentially localize at a specific target, SBI turned to an exciting new technology, XStamp Pro, which works on already purified EVs—no cloning or transfection required. Simply incubate purified EVs with the XStamp Pro ScFv for one-hour and then isolate your target-specific EVs.

How It Works
Engineer target specificity into already-purified EVs XStamp Pro takes advantage of the same EV surface-directing protein used in our original XStamp products but skips the expression vector and the transfection step for a faster, simpler workflow. You get an already purified peptide consisting of the target-specific ScFv fused to the EV surface-directing protein—the XStamp Pro ScFv. Upon incubation with isolated EVs, the XStamp Pro ScFv self-inserts into EV membranes creating your target-specific EVs (Figure 1). Incubation is complete in 1 hour, after which the target-specific EVs can be isolated using the included ExoQuick-TC reagent or other isolation method of your choice, and then used to deliver cargo.