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C6 B. burgdorferi (Lyme) ELISA Kit - A new standard in accuracy

The C6 Kit

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Immunetics introduces a new B. burgdorferi (Lyme) ELISA kit* based on the C6 peptide of the VlsE protein, offering a quantum leap forward in accuracy.

For In Vitro Diagnostic Use

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Highlights pix.GIF (45 bytes)
· Accurate:

▪ Near Western Blot accuracy in an ELISA

· Sensitive

▪ More sensitive in detection of antibodies in early and late Lyme disease than competing kits

· Specific

▪ Eliminates vast majority of other ELISA’s false positives at the screening step
▪ Significantly higher specificity than competing ELISAs

· Broadly applicable

▪ Detects infection with all strains of Borrelia which cause Lyme disease, including European strains B. garinii and B. afzelii

· Convenient

▪ 1 hour turnaround time
▪ Breakaway strips for any number of samples
▪ Ready-to-use reagents, controls included

· Cost-effective

▪ Greatly reduces the number of samples sent for expensive confirmatory testing

Principle

This ELISA is based on the C6 peptide antigen*, a 26 amino acid sequence of the surface protein VlsE. This peptide has been shown to represent the immunodominant portion of VlsE, containing substantially all of the antigenic reactivity of the whole protein. Recent studies have shown that the C6 peptide is a sensitive and specific marker for antibody to B. burgdorferi sensu lato and for the serodiagnosis of Lyme disease.

As the C6 antigen represents a defined sequence within the protein, cross-reactivity with antigens found in other organisms is greatly reduced.

Methodology

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C6 ELISA results match Western Blot results:

Western Blot Pos Neg

C6 Pos/Eqv 95% 1.7%
Neg 1.1% 2.2%

n = 180
Concordance with WB = 97.2%

Components


Kit information:

Cat # DK-E352-096

Contains a coated microwell plate with 96 breakaway wells, controls and all reagents necessary to perform the assay.
* For in vitro diagnostic use