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Western blot is a laboratory technique used to detect specific proteins in a sample of tissue. It's often used in the fields of biochemistry and molecular biology to identify and analyze proteins. The process involves separating proteins by size using gel electrophoresis, transferring them to a membrane, and then applying specific antibodies that will bind to the protein of interest. The resulting "blot" can be visualized using a chemiluminescent or fluorescent detection method, allowing researchers to identify the presence and quantity of the target protein in the sample. Western blot is a useful tool for studying the function and regulation of proteins, and it's commonly used in research and diagnostic applications.