Chimeric Anti EBOV (H113C6FR1)

Catalog #:

0201-023

Immunogen:

Venezuelan equine encephalitis virus replicons encoding Zaire ebolavirus (EBOV) glycoprotein (GP) were used to generate the original mouse monoclonal antibody.

Description:

A murine / human chimeric IgG produced in N. benthamiana and is reactive to EBOV GP. The antibody detects GP in virus-like particles (VLP) and recombinant GP without the transmembrane region (rGPΔTM).

Supplied:

100 µg is supplied at a concentration of 2.456 mg/mL. No preservative is added.

Purification:

Antibody is purified using immobilized protein A.

Clonality:

Murine variable, human constant of the IgG1 isotype.

Relevance:

The antibody can be used for detection of EBOV GP in ELISA. A mixture of all three anti-EBOV GP chimeric antibodies was protective against lethal challenge in a nonhuman primate study (Olinger et al. PNAS 2012, vol. 109, no. 44, 18030-18035).

ELISA:

Assay-dependent dilution

WB:

Not recommended because this antibody recognizes a conformational epitope.

Cross Reactivity:

Cross-reactivity was observed against Sudan ebolavirus (SUDV) VLP and especially against SUDV rGPΔTM. No cross-reactivity against Marburg Virus (MARV) VLP or rGPΔTM.

Storage:

2-3 weeks +4oC, -20◦C long term