Description

Anti-Phosphorilated Histone H2AX

Quality Level

100

biological source

mouse

antibody form

affinity purified immunoglobulin

clone

JBW301, monoclonal

species reactivity

vertebrates

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate^®

technique(s)

ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NP_002096

General description

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Specificity

Recognizes Histone H2A.X phosphorylated at Ser139.

Immunogen

peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X

Application

Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) also known as H2AXS139p.

Research Sub Category
Histones

Research Category
Epigenetics & Nuclear Function

Additional Referenced Applications:

Immunohistochemistry Analysis: A representative lot detected Histone H2A.X (pSer139) in RNF168-WT and RNF 168-SA/SEKI mice lung tissue sections (Paraffin). (Xe, X., et al. (2015) Nat. Cell Biol. 20 (3); 320-331).
Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.

View AllClose