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Miniblotter® Systems
for BioRad Criterion®
PreCast Gels |
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Overview
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The
Miniblotter®instruments
enable you to perform
multiple antibody or probe incubations on a single membrane, using
microliter volumes of reagent. Typical applications include
monoclonal antibody
screening on western blots, animal and human serum screening, and DNA
probe hybridizations. Sample processing is streamlined through use of
the Washing Manifold, which enables washing of all sample lanes
simultaneously. Reactions can be detected by all conventional
methods, including enzyme conjugates, chemiluminescence and radiolabels.
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Procedure |
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A blot or other membrane is inserted into
the Miniblotter.
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Antibody or probe samples are pipetted
into the Miniblotter's parallel channels and incubated on the membrane.
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Samples
are aspirated and the membrane is then washed using the Washing Manifold.
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Detection
reagent is pipetted into the Miniblotter channels and incubated on the
membrane.
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Reagent
is aspirated and the membrane is washed again using the Washing Manifold.
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The
membrane is removed from the Miniblotter and incubated with the
development reagent or substrate to reveal bands.
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Results
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Miniblotter 18
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- 18 channels (samples) per blot.
- Unique channel design allows testing of
sample sizes from 110 to 325µl
per
channel.
- Channels of the MN18 align with the wells
on the corresponding BioRad Criterion®
gel:
- Channel dimension: 2.4 mm x 8 cm
- Blot width covered: 13.5 cm
- Washing Manifold (F2) available for simultaneously washing
of all channels.
- Results on a single membrane are compared easily without
switching or breaking of strips.
- Instruments function with all detection methods (i.e.,
enzymatic, radioactive, chemiluminescence).
- Catalog # MN18
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Miniblotter 26
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- 26
channels (samples) per blot.
- Unique channel design allows testing of
sample sizes from 110 to 325µl
per
channel.
- Channels of the MN26 align with the wells
on the corresponding BioRad Criterion®
gel:
- Channel dimension: 2.4 mm x 8 cm
- Blot width covered: 13.5 cm
- Washing Manifold (F2) available for simultaneously washing
of all channels.
- Results on a single membrane are compared easily without
switching or breaking of strips.
- Instruments function with all detection methods (i.e.,
enzymatic, radioactive, chemiluminescence).
- Catalog # MN26
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