The Miniblotter™ instruments enable you to perform multiple antibody or probe incubations on a single membrane, using microliter volumes of reagent.  Typical applications include monoclonal antibody screening on western blots, animal and human serum screening, and DNA probe hybridizations.  Sample processing is streamlined through use of the Washing Manifold, which enables washing of all sample lanes simultaneously.  Reactions can be detected by all conventional methods, including enzyme conjugates, chemiluminescence and radiolabels.

                         Procedure

1. A blot or other membrane is inserted into the Miniblotter.
2. Antibody or probe samples are pipetted into the Miniblotter's parallel channels and incubated on the membrane.
3. Samples are aspirated and the membrane is then washed using the Washing Manifold.
4. Detection reagent is pipetted into the Miniblotter channels and incubated on the membrane.
5. Reagent is aspirated and the membrane is washed again using the Washing Manifold.
6. The membrane is removed from the Miniblotter and incubated with the development reagent or substrate to reveal bands.
7. Result

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• 28 channels (samples) per blot.
• Holds up to 50µl of antibody or probe solution per channel.
• Dimensions compatible with most standard gel electrophoresis chambers:
• Channel dimension: 1.5 mm x 5.5 cm
• Blot width covered: 8.3 cm
• Ideal for hybridoma screening from 96-well microplates. Hybridoma supernatants can be transferred directly into Miniblotter 28 channels using an 8-channel automatic pipette.
• Washing Manifold (F1) available for simultaneously washing of all channels.
• Results on a single membrane are compared easily without switching or breaking of strips.
• Instruments function with all detection methods (i.e., enzymatic, radioactive, chemiluminescence).
• Cat # MN28SL
• Cat # MN100-28SL(w/ washing manifold)

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• Same as Single Unit except there are two sets of 28 channels so that two membrane blots can be placed between the Top and Bottom Plates allowing for the processing of up to 56 samples (28x2).

• Cat # MN28
• Cat # MN100-28 (w/ washing manifold)

• 20 channels (samples) per blot.
• Holds up to 350µl of antibody or probe solution per channel.
• Dimensions compatible with most minigel electrophoresis chambers:
• Channel dimension: 3.0mm x 5.5cm
• Blot width covered: 8.3cm
• Greater volume per channel for highest sensitivity detection of low affinity and low titer antibodies.
• Washing Manifold (F1) available for simultaneously washing of all channels.
• Results on a single membrane are compared easily without switching or breaking of strips.
• Instruments function with all detection methods (i.e., enzymatic, radioactive, chemiluminescence).
• Cat # MN20SL
• Cat # NM 100-20SL (w/ washing manifold)

Miniblotter 16 - Dual Unit  

     click image to enlarge

• Similar to Miniblotter 20 - Single Unit, except that there are two sets of 16 channels so that two membrane blots can be placed between the Top and Bottom Plates allowing for the processing of up to 32 samples (16x2).

• Cat # MN16
• Cat # MN 100-16 (w/ washing manifold)

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