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Miniblotter Systems
for Regular Gel Processing |
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Overview
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The
Miniblotter™ instruments enable you to perform
multiple antibody or probe incubations on a single membrane, using
microliter volumes of reagent. Typical applications include
monoclonal antibody
screening on western blots, animal and human serum screening, and DNA
probe hybridizations. Sample processing is streamlined through use of
the Washing Manifold, which enables washing of all sample lanes
simultaneously. Reactions can be detected by all conventional
methods, including enzyme conjugates, chemiluminescence and radiolabels.
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Procedure |
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- A blot or other membrane is inserted into
the Miniblotter.
- Antibody or probe samples are pipetted
into the Miniblotter's parallel channels and incubated on the membrane.
- Samples
are aspirated and the membrane is then washed using the Washing Manifold.
- Detection
reagent is pipetted into the Miniblotter channels and incubated on the
membrane.
- Reagent
is aspirated and the membrane is washed again using the Washing Manifold.
- The
membrane is removed from the Miniblotter and incubated with the
development reagent or substrate to reveal bands.
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Results
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Miniblotter 45 Unit
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- 45 channels (samples) per blot.
- Holds up to 110µl of antibody or probe solution per
channel.
- Dimensions compatible with most standard gel
electrophoresis chambers:
- Channel dimension: 2.0 mm x 13 cm
- Blot width covered: 13 cm
- Washing Manifold (F2) available for simultaneously washing
of all channels.
- Results on a single membrane are compared easily without
switching or breaking of strips.
- Instruments function with all detection methods (i.e.,
enzymatic, radioactive, chemiluminescence).
- Combs available for gel electrophoresis to line up
Miniblotter channels with electrophoresis lane
- Cat # MN45
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Miniblotter 25 Unit
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- 25
channels (samples) per blot.
- Holds up to 250µl of antibody or probe solution per
channel.
- Dimensions compatible with most standard gel electrophoresis
chambers:
- Channel dimension: 4.0 mm x 13 cm
- Blot width covered: 13 cm
- Greater volume per channel for highest sensitivity detection
of low affinity and low titer antibodies.
- Washing Manifold (F2) available for simultaneously washing
of all channels.
- Results on a single membrane are compared easily without
switching or breaking of strips.
- Instruments function with all detection methods (i.e.,
enzymatic, radioactive, chemiluminescence).
- Cat # MN25
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