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     Cell Adhesion Flow Chamber

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Description
of Use

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The Immunetics Cell Adhesion Flow Chamber is designed for performing dynamic cell adhesion assays in a laminar flow environment. The transparent acrylic device permits the study of interactions between cells immobilized on a microscope slide and cells in the fluid phase. Immobilized cells are grown on a detachable slide culture vessel. The Flow Chamber is mounted on an inverted microscope, cells in suspension are pumped through the chamber and cell-cell interactions are monitored in real time by video microscopy. The design solves several problems, including establishment of true laminar flow without turbulence, and leak-proof, reversible sealing of the culture slide to the chamber by vacuum. Applications for the Flow Chamber include studies of cell adhesion and cell-cell interactions in immunology and cell biology.
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Technical Specifications

pix.GIF (45 bytes) Accepts any microscope slide - cell culture vessel
  • Depth of flow deck = 250 µ, volume = 50 µl
  • Shear stress range 0.3 - 1.8 dyne/cm2
  • Compatible with syringe pumps for controlling liquid flow
  • Mounts on a microscope stage equipped to manipulate 96-well plates
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 Unique Features pix.GIF (45 bytes)
  • Rapid stability of laminar flow with minimal turbulence
  • Leak-proof vacuum sealing between slide and chamber
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pix.GIF (45 bytes)Schematic of the Flow Chamber showing top and side views. Vacuum is applied between the two O-ring gaskets to anchor the slide, and is isolated from the flow deck by the gaskets. Cells in fluid phase are introduced via inlet and outlet ports. A series of five apertures in inlet and outlet manifolds facilitates rapid stabilization of laminar flow. External vacuum maintains a constant gap between the culture slide (crosshatched) and the flow deck. All liquid and vacuum fittings accept luer connectors. (See diagram below)

ordering information: catalog # CAF10

Immunetics, Inc.
 27 Drydock Ave, 6th Floor, Boston MA 02210 USA
 Tel: 617.896.9100    Toll-Free: 800.227.4765
 Fax: 617.896.9110    email: info@immunetics.com

© Copyright 1997 Immunetics, Inc. All Rights Reserved.
Miniblot, Miniblotter and Immunetics are registered trademarks, and MiniSlot, and QualiCode, are trademarks of Immunetics, Inc.